chemidoc imaging software (Bio-Rad)
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Chemidoc Imaging Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 22492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mp+software/ChemiDoc+MP+Imaging+System/10__1158_slash_2767___9764__crc___25___0701-104-57-60
Average 99 stars, based on 22492 article reviews
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Software:Article Title: ScFv T1 Protects Against Mitochondrial Damage of SH-SY5Y Cells Caused by Extracellular Tau Aggregates. Article Snippet: The membranes were then incubated with HRP-conjugated secondary antibodies (goat anti-mouse HRP-conjugated, ABclonal, Wuhan, China, AS003; goat anti-rabbit HRP-conjugated, Proteintech Cat# SA00001-2) for 1 h at RT and washed 3 times with TBST for 15 min each time. .. Finally, the bands were visualized with a chemiluminescent HRP substrate (Millipore, Cat# P90719) and analyzed using a ChemiDocTM XRS+ system with Article Title: DGAT1 Drives Racially Divergent Fibroblast Activation via ERK1/2-Dependent Tumorigenic Signaling in Prostate Cancer Article Snippet: .. C R C - 2 5 - 0 7 0 1 / 3 7 9 3 7 3 3 / c r c - 2 5 - 0 7 0 1 . p d f b y g u e s t o n 1 5 M a y 2 0 2 6 array were detected using Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease. Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by Article Title: Dynamic Regulation of Atg4 Protease and Autophagy by Dithiothreitol and Iron in Alternaria alternata Article Snippet: Proteins on PVDF were incubated with a rabbit anti-GFP antibody (1:5000) overnight at 4 °C and reacted with an HRP-conjugated goat anti-rabbit IgG (1:10,000, Jackson ImmunoResearch, West Grove, PA, U.S.A.) and LumiFlash Prime Chemiluminescent Substrate, HRP (Visual Protein, Taipei, Taiwan). .. Chemiluminescence signals were acquired using the Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo. Article Snippet: .. For protein detection Western Lightning Plus ECL solution (Perkin-Elmer, NEL105001EA) or Clarity Western ECL Substrate (Bio-Rad, 1705061) was used and analyzed with the Article Title: Myo1e/f regulate phagocytic podosomes to promote efficient cup closure in macrophages Article Snippet: After incubating the membrane with HRP-tagged secondary antibodies, blot was developed using ECL reagent (BioRad, 1705060). .. The ECL signal was visualized using a Imaging:Article Title: DGAT1 Drives Racially Divergent Fibroblast Activation via ERK1/2-Dependent Tumorigenic Signaling in Prostate Cancer Article Snippet: .. C R C - 2 5 - 0 7 0 1 / 3 7 9 3 7 3 3 / c r c - 2 5 - 0 7 0 1 . p d f b y g u e s t o n 1 5 M a y 2 0 2 6 array were detected using Article Title: ZDHHC11-mediated palmitoylation of viral envelope protein restricts dengue virus replication: implication for the development of universal anti-flavivirus therapeutic strategies. Article Snippet: Membranes were blocked with Western Blocking Buffer (Proteintech, PR2034, USA) at room temperature for 15 min, then incubated overnight at 4°C with primary antibodies: anti-GAPDH (Proteintech, 60004-1-ig, 1:50000); anti-DENV Envelope (GeneTex, GTX127277, 1:10000); anti-DENV Capsid (GeneTex, GTX639659, 1:1000); anti-DENV prM (GeneTex, GTX640942, 1:1000); anti-ZIKV NS3 (GeneTex, GTX133309, 1:4000); anti-Flag antibody (Proteintech, 66008-4-ig, 1:10000); anti-HA antibody (Sigma, H6908, 1:1000). .. After three washes with TBST (10 min each), membranes were incubated with multi-rAb HRP-goat anti-rabbit/mouse recombinant secondary antibody (H+L) (Proteintech, RGAR001 for rabbit IgG; RGARM001 for mouse IgG; both 1:5000) at room temperature for 1 h. Protein bands were visualized using Article Title: The Antipsychotic Aripiprazole Induces Cytotoxicity in Bladder Cancer Cells While Preserving Urothelial and Bladder Function. Article Snippet: .. Signal intensity was captured using a Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease. Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by Article Title: Dynamic Regulation of Atg4 Protease and Autophagy by Dithiothreitol and Iron in Alternaria alternata Article Snippet: Proteins on PVDF were incubated with a rabbit anti-GFP antibody (1:5000) overnight at 4 °C and reacted with an HRP-conjugated goat anti-rabbit IgG (1:10,000, Jackson ImmunoResearch, West Grove, PA, U.S.A.) and LumiFlash Prime Chemiluminescent Substrate, HRP (Visual Protein, Taipei, Taiwan). .. Chemiluminescence signals were acquired using the Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo. Article Snippet: .. For protein detection Western Lightning Plus ECL solution (Perkin-Elmer, NEL105001EA) or Clarity Western ECL Substrate (Bio-Rad, 1705061) was used and analyzed with the Article Title: Myo1e/f regulate phagocytic podosomes to promote efficient cup closure in macrophages Article Snippet: After incubating the membrane with HRP-tagged secondary antibodies, blot was developed using ECL reagent (BioRad, 1705060). .. The ECL signal was visualized using a Incubation:Article Title: ZDHHC11-mediated palmitoylation of viral envelope protein restricts dengue virus replication: implication for the development of universal anti-flavivirus therapeutic strategies. Article Snippet: Membranes were blocked with Western Blocking Buffer (Proteintech, PR2034, USA) at room temperature for 15 min, then incubated overnight at 4°C with primary antibodies: anti-GAPDH (Proteintech, 60004-1-ig, 1:50000); anti-DENV Envelope (GeneTex, GTX127277, 1:10000); anti-DENV Capsid (GeneTex, GTX639659, 1:1000); anti-DENV prM (GeneTex, GTX640942, 1:1000); anti-ZIKV NS3 (GeneTex, GTX133309, 1:4000); anti-Flag antibody (Proteintech, 66008-4-ig, 1:10000); anti-HA antibody (Sigma, H6908, 1:1000). .. After three washes with TBST (10 min each), membranes were incubated with multi-rAb HRP-goat anti-rabbit/mouse recombinant secondary antibody (H+L) (Proteintech, RGAR001 for rabbit IgG; RGARM001 for mouse IgG; both 1:5000) at room temperature for 1 h. Protein bands were visualized using Recombinant:Article Title: ZDHHC11-mediated palmitoylation of viral envelope protein restricts dengue virus replication: implication for the development of universal anti-flavivirus therapeutic strategies. Article Snippet: Membranes were blocked with Western Blocking Buffer (Proteintech, PR2034, USA) at room temperature for 15 min, then incubated overnight at 4°C with primary antibodies: anti-GAPDH (Proteintech, 60004-1-ig, 1:50000); anti-DENV Envelope (GeneTex, GTX127277, 1:10000); anti-DENV Capsid (GeneTex, GTX639659, 1:1000); anti-DENV prM (GeneTex, GTX640942, 1:1000); anti-ZIKV NS3 (GeneTex, GTX133309, 1:4000); anti-Flag antibody (Proteintech, 66008-4-ig, 1:10000); anti-HA antibody (Sigma, H6908, 1:1000). .. After three washes with TBST (10 min each), membranes were incubated with multi-rAb HRP-goat anti-rabbit/mouse recombinant secondary antibody (H+L) (Proteintech, RGAR001 for rabbit IgG; RGARM001 for mouse IgG; both 1:5000) at room temperature for 1 h. Protein bands were visualized using Expressing:Article Title: The Antipsychotic Aripiprazole Induces Cytotoxicity in Bladder Cancer Cells While Preserving Urothelial and Bladder Function. Article Snippet: .. Signal intensity was captured using a Staining:Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease. Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by Labeling:Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease. Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by Western Blot:Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo. Article Snippet: .. For protein detection Western Lightning Plus ECL solution (Perkin-Elmer, NEL105001EA) or Clarity Western ECL Substrate (Bio-Rad, 1705061) was used and analyzed with the |
