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chemidoc imaging software  (Bio-Rad)


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    Structured Review

    Bio-Rad chemidoc imaging software
    Chemidoc Imaging Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 22492 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/ChemiDoc+MP+Imaging+System/10__1158_slash_2767___9764__crc___25___0701-104-57-60
    Average 99 stars, based on 22492 article reviews
    chemidoc imaging software - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Software:

    Article Title: ScFv T1 Protects Against Mitochondrial Damage of SH-SY5Y Cells Caused by Extracellular Tau Aggregates.
    Article Snippet: The membranes were then incubated with HRP-conjugated secondary antibodies (goat anti-mouse HRP-conjugated, ABclonal, Wuhan, China, AS003; goat anti-rabbit HRP-conjugated, Proteintech Cat# SA00001-2) for 1 h at RT and washed 3 times with TBST for 15 min each time. .. Finally, the bands were visualized with a chemiluminescent HRP substrate (Millipore, Cat# P90719) and analyzed using a ChemiDocTM XRS+ system with Image LabTM Software version 3.0 (Bio-Rad, Hercules, CA, USA, ChemiDoc MP) [24]. ..

    Article Title: DGAT1 Drives Racially Divergent Fibroblast Activation via ERK1/2-Dependent Tumorigenic Signaling in Prostate Cancer
    Article Snippet: .. C R C - 2 5 - 0 7 0 1 / 3 7 9 3 7 3 3 / c r c - 2 5 - 0 7 0 1 . p d f b y g u e s t o n 1 5 M a y 2 0 2 6 array were detected using ChemiDoc Imaging software (Bio-Rad), and signal intensity was quantified using the Fiji plugin in ImageJ software (RRID:SCR_002285, Version 1.53o) 9,25. ..

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by ChemiDoc MP Imaging System (Bio-Rad). aSyn bands were identified and quantified using Image Lab software (Bio-Rad, RRID:SCR_014210), including normalization to total protein from stain-free labeling. ..

    Article Title: Dynamic Regulation of Atg4 Protease and Autophagy by Dithiothreitol and Iron in Alternaria alternata
    Article Snippet: Proteins on PVDF were incubated with a rabbit anti-GFP antibody (1:5000) overnight at 4 °C and reacted with an HRP-conjugated goat anti-rabbit IgG (1:10,000, Jackson ImmunoResearch, West Grove, PA, U.S.A.) and LumiFlash Prime Chemiluminescent Substrate, HRP (Visual Protein, Taipei, Taiwan). .. Chemiluminescence signals were acquired using the ChemiDoc MP imaging system (Bio-Rad) and ImageLab software (version 6.1.0). ..

    Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.
    Article Snippet: .. For protein detection Western Lightning Plus ECL solution (Perkin-Elmer, NEL105001EA) or Clarity Western ECL Substrate (Bio-Rad, 1705061) was used and analyzed with the ChemiDoc MP Imaging System and ImageLab 6.1 software (Bio-Rad). .. Quantification was done with ImageJ v.1.54 and normalized to β-actin.

    Article Title: Myo1e/f regulate phagocytic podosomes to promote efficient cup closure in macrophages
    Article Snippet: After incubating the membrane with HRP-tagged secondary antibodies, blot was developed using ECL reagent (BioRad, 1705060). .. The ECL signal was visualized using a Chemidoc MP imaging system (Bio-Rad) and Image Lab analysis software (Bio-Rad). .. The following primary antibodies were used: rabbit anti-myo1e (Sigma-Aldrich, HPA023886, Fig. S1A), homemade rabbit anti-myo1e (RRID AB_2909514, Fig. S1B), mouse anti-myo1f (Santa-Cruz, sc-376534).

    Imaging:

    Article Title: DGAT1 Drives Racially Divergent Fibroblast Activation via ERK1/2-Dependent Tumorigenic Signaling in Prostate Cancer
    Article Snippet: .. C R C - 2 5 - 0 7 0 1 / 3 7 9 3 7 3 3 / c r c - 2 5 - 0 7 0 1 . p d f b y g u e s t o n 1 5 M a y 2 0 2 6 array were detected using ChemiDoc Imaging software (Bio-Rad), and signal intensity was quantified using the Fiji plugin in ImageJ software (RRID:SCR_002285, Version 1.53o) 9,25. ..

    Article Title: ZDHHC11-mediated palmitoylation of viral envelope protein restricts dengue virus replication: implication for the development of universal anti-flavivirus therapeutic strategies.
    Article Snippet: Membranes were blocked with Western Blocking Buffer (Proteintech, PR2034, USA) at room temperature for 15 min, then incubated overnight at 4°C with primary antibodies: anti-GAPDH (Proteintech, 60004-1-ig, 1:50000); anti-DENV Envelope (GeneTex, GTX127277, 1:10000); anti-DENV Capsid (GeneTex, GTX639659, 1:1000); anti-DENV prM (GeneTex, GTX640942, 1:1000); anti-ZIKV NS3 (GeneTex, GTX133309, 1:4000); anti-Flag antibody (Proteintech, 66008-4-ig, 1:10000); anti-HA antibody (Sigma, H6908, 1:1000). .. After three washes with TBST (10 min each), membranes were incubated with multi-rAb HRP-goat anti-rabbit/mouse recombinant secondary antibody (H+L) (Proteintech, RGAR001 for rabbit IgG; RGARM001 for mouse IgG; both 1:5000) at room temperature for 1 h. Protein bands were visualized using Bio-Rad ChemiDoc MP Imaging System (USA) and densitometrically analyzed with ImageJ software (NIH, USA). ..

    Article Title: The Antipsychotic Aripiprazole Induces Cytotoxicity in Bladder Cancer Cells While Preserving Urothelial and Bladder Function.
    Article Snippet: .. Signal intensity was captured using a Bio- Rad ChemiDoc MP imaging system, and relative protein expression was quantified by densitometric analysis of each spot using ImageJ software (v2.16.0). ..

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by ChemiDoc MP Imaging System (Bio-Rad). aSyn bands were identified and quantified using Image Lab software (Bio-Rad, RRID:SCR_014210), including normalization to total protein from stain-free labeling. ..

    Article Title: Dynamic Regulation of Atg4 Protease and Autophagy by Dithiothreitol and Iron in Alternaria alternata
    Article Snippet: Proteins on PVDF were incubated with a rabbit anti-GFP antibody (1:5000) overnight at 4 °C and reacted with an HRP-conjugated goat anti-rabbit IgG (1:10,000, Jackson ImmunoResearch, West Grove, PA, U.S.A.) and LumiFlash Prime Chemiluminescent Substrate, HRP (Visual Protein, Taipei, Taiwan). .. Chemiluminescence signals were acquired using the ChemiDoc MP imaging system (Bio-Rad) and ImageLab software (version 6.1.0). ..

    Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.
    Article Snippet: .. For protein detection Western Lightning Plus ECL solution (Perkin-Elmer, NEL105001EA) or Clarity Western ECL Substrate (Bio-Rad, 1705061) was used and analyzed with the ChemiDoc MP Imaging System and ImageLab 6.1 software (Bio-Rad). .. Quantification was done with ImageJ v.1.54 and normalized to β-actin.

    Article Title: Myo1e/f regulate phagocytic podosomes to promote efficient cup closure in macrophages
    Article Snippet: After incubating the membrane with HRP-tagged secondary antibodies, blot was developed using ECL reagent (BioRad, 1705060). .. The ECL signal was visualized using a Chemidoc MP imaging system (Bio-Rad) and Image Lab analysis software (Bio-Rad). .. The following primary antibodies were used: rabbit anti-myo1e (Sigma-Aldrich, HPA023886, Fig. S1A), homemade rabbit anti-myo1e (RRID AB_2909514, Fig. S1B), mouse anti-myo1f (Santa-Cruz, sc-376534).

    Incubation:

    Article Title: ZDHHC11-mediated palmitoylation of viral envelope protein restricts dengue virus replication: implication for the development of universal anti-flavivirus therapeutic strategies.
    Article Snippet: Membranes were blocked with Western Blocking Buffer (Proteintech, PR2034, USA) at room temperature for 15 min, then incubated overnight at 4°C with primary antibodies: anti-GAPDH (Proteintech, 60004-1-ig, 1:50000); anti-DENV Envelope (GeneTex, GTX127277, 1:10000); anti-DENV Capsid (GeneTex, GTX639659, 1:1000); anti-DENV prM (GeneTex, GTX640942, 1:1000); anti-ZIKV NS3 (GeneTex, GTX133309, 1:4000); anti-Flag antibody (Proteintech, 66008-4-ig, 1:10000); anti-HA antibody (Sigma, H6908, 1:1000). .. After three washes with TBST (10 min each), membranes were incubated with multi-rAb HRP-goat anti-rabbit/mouse recombinant secondary antibody (H+L) (Proteintech, RGAR001 for rabbit IgG; RGARM001 for mouse IgG; both 1:5000) at room temperature for 1 h. Protein bands were visualized using Bio-Rad ChemiDoc MP Imaging System (USA) and densitometrically analyzed with ImageJ software (NIH, USA). ..

    Recombinant:

    Article Title: ZDHHC11-mediated palmitoylation of viral envelope protein restricts dengue virus replication: implication for the development of universal anti-flavivirus therapeutic strategies.
    Article Snippet: Membranes were blocked with Western Blocking Buffer (Proteintech, PR2034, USA) at room temperature for 15 min, then incubated overnight at 4°C with primary antibodies: anti-GAPDH (Proteintech, 60004-1-ig, 1:50000); anti-DENV Envelope (GeneTex, GTX127277, 1:10000); anti-DENV Capsid (GeneTex, GTX639659, 1:1000); anti-DENV prM (GeneTex, GTX640942, 1:1000); anti-ZIKV NS3 (GeneTex, GTX133309, 1:4000); anti-Flag antibody (Proteintech, 66008-4-ig, 1:10000); anti-HA antibody (Sigma, H6908, 1:1000). .. After three washes with TBST (10 min each), membranes were incubated with multi-rAb HRP-goat anti-rabbit/mouse recombinant secondary antibody (H+L) (Proteintech, RGAR001 for rabbit IgG; RGARM001 for mouse IgG; both 1:5000) at room temperature for 1 h. Protein bands were visualized using Bio-Rad ChemiDoc MP Imaging System (USA) and densitometrically analyzed with ImageJ software (NIH, USA). ..

    Expressing:

    Article Title: The Antipsychotic Aripiprazole Induces Cytotoxicity in Bladder Cancer Cells While Preserving Urothelial and Bladder Function.
    Article Snippet: .. Signal intensity was captured using a Bio- Rad ChemiDoc MP imaging system, and relative protein expression was quantified by densitometric analysis of each spot using ImageJ software (v2.16.0). ..

    Staining:

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by ChemiDoc MP Imaging System (Bio-Rad). aSyn bands were identified and quantified using Image Lab software (Bio-Rad, RRID:SCR_014210), including normalization to total protein from stain-free labeling. ..

    Labeling:

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by ChemiDoc MP Imaging System (Bio-Rad). aSyn bands were identified and quantified using Image Lab software (Bio-Rad, RRID:SCR_014210), including normalization to total protein from stain-free labeling. ..

    Western Blot:

    Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.
    Article Snippet: .. For protein detection Western Lightning Plus ECL solution (Perkin-Elmer, NEL105001EA) or Clarity Western ECL Substrate (Bio-Rad, 1705061) was used and analyzed with the ChemiDoc MP Imaging System and ImageLab 6.1 software (Bio-Rad). .. Quantification was done with ImageJ v.1.54 and normalized to β-actin.



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    <t>Label‐free</t> <t>MP‐SPR</t> quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.
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    Image Search Results


    Label‐free MP‐SPR quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.

    Journal: Journal of Extracellular Vesicles

    Article Title: Hyaluronic Acid Decoration Facilitates CD44‐Mediated Targeting and Alters Protein Corona Formation of Extracellular Vesicles

    doi: 10.1002/jev2.70263

    Figure Lengend Snippet: Label‐free MP‐SPR quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.

    Article Snippet: The SPR reflectance spectrum was recorded between 58° and 77° with a scanning time of approximately 2.0 s. Data were exported in ASCII format with MP‐SPR Navi Data Viewer 6.7.0.1 software (BioNavis), and SPR peak angular positions were determined using the weighted centroid method.

    Techniques: Comparison, Microscopy, Standard Deviation

    MP‐SPR based CD9‐antibody capture of EVs and plasma‐derived corona formation. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) and the ratio of the two wavelengths (dots) from CD9‐antibody captured MCF7‐EVs (A) and HAS3‐EVs (B) over 30‐min injections of EVs ( n = 3, STDEV). After EV capture, EV‐depleted plasma was injected to the system, causing a sharp increase in the SPR‐signal, followed by sharp decrease after washing with PBS (indicated with arrows). The corona refractive index and thickness were calculated from the remaining tail signal. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) for plasma injection and protein corona formation on MCF7‐EVs (C) and HAS3‐EVs (D) ( n = 3, STDEV). The average ratio of the signals measured at 670 and 785 nm for the dual‐wavelength method to calculate the EV diameters, and refractive index (RI) and thickness of the protein corona determined with LayerSolver are shown in the table. EV, extracellular vesicle; HA, hyaluronic acid.

    Journal: Journal of Extracellular Vesicles

    Article Title: Hyaluronic Acid Decoration Facilitates CD44‐Mediated Targeting and Alters Protein Corona Formation of Extracellular Vesicles

    doi: 10.1002/jev2.70263

    Figure Lengend Snippet: MP‐SPR based CD9‐antibody capture of EVs and plasma‐derived corona formation. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) and the ratio of the two wavelengths (dots) from CD9‐antibody captured MCF7‐EVs (A) and HAS3‐EVs (B) over 30‐min injections of EVs ( n = 3, STDEV). After EV capture, EV‐depleted plasma was injected to the system, causing a sharp increase in the SPR‐signal, followed by sharp decrease after washing with PBS (indicated with arrows). The corona refractive index and thickness were calculated from the remaining tail signal. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) for plasma injection and protein corona formation on MCF7‐EVs (C) and HAS3‐EVs (D) ( n = 3, STDEV). The average ratio of the signals measured at 670 and 785 nm for the dual‐wavelength method to calculate the EV diameters, and refractive index (RI) and thickness of the protein corona determined with LayerSolver are shown in the table. EV, extracellular vesicle; HA, hyaluronic acid.

    Article Snippet: The SPR reflectance spectrum was recorded between 58° and 77° with a scanning time of approximately 2.0 s. Data were exported in ASCII format with MP‐SPR Navi Data Viewer 6.7.0.1 software (BioNavis), and SPR peak angular positions were determined using the weighted centroid method.

    Techniques: Clinical Proteomics, Derivative Assay, Injection, Refractive Index