Review



chemidoc mp multimode imager  (Bio-Rad)


Bioz Verified Symbol Bio-Rad is a verified supplier
Bioz Manufacturer Symbol Bio-Rad manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 99

    Structured Review

    Bio-Rad chemidoc mp multimode imager
    Chemidoc Mp Multimode Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2463 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/Image+Lab+Touch+Software+for+ChemiDoc+Touch+Imager+Version+2%2E3/pmc13621846-284-6-10
    Average 99 stars, based on 2463 article reviews
    chemidoc mp multimode imager - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Expansion and optimization of the auxin-inducible degron 2 (AID2) system in Candida pathogens
    Article Snippet: Immunoblots using HRP were developed with Clarity Western ECL Substrate (Bio-Rad, 170-5060). .. All immunoblots were imaged on a ChemiDoc MP multimode imager (Bio-Rad). .. Band intensities were quantified using ImageLab software (Bio-Rad).

    Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.
    Article Snippet: .. For protein detection Western Lightning Plus ECL solution (Perkin-Elmer, NEL105001EA) or Clarity Western ECL Substrate (Bio-Rad, 1705061) was used and analyzed with the ChemiDoc MP Imaging System and ImageLab 6.1 software (Bio-Rad). .. Quantification was done with ImageJ v.1.54 and normalized to β-actin.

    Imaging:

    Article Title: FANCB as a prognostic biomarker and therapeutic target in hepatocellular carcinoma with insights from pan-cancer analysis and experimental validation
    Article Snippet: .. Protein bands were visualized using the Bio-Rad ChemiDoc MP Imaging System and analyzed by Image J software. .. The primary antibodies used in this experiment include anti-FANCB rabbit polyclonal antibody (D263885, BBI Life Sciences Corporation, Shanghai, China) and GAPDH monoclonal antibody (60004-1-Ig, Proteintech, Wuhan, China).

    Article Title: Portulaca oleracea L.-derived extracellular vesicle-like particles reprogram intestinal ILCs to alleviate inflammatory bowel disease and colitis-associated colorectal cancer
    Article Snippet: .. The images were captured with the ChemiDoc MP Imaging System (USA, Bio-Rad) and analyzed with Image Lab Software. ..

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by ChemiDoc MP Imaging System (Bio-Rad). aSyn bands were identified and quantified using Image Lab software (Bio-Rad, RRID:SCR_014210), including normalization to total protein from stain-free labeling. ..

    Article Title: Dynamic Regulation of Atg4 Protease and Autophagy by Dithiothreitol and Iron in Alternaria alternata
    Article Snippet: Proteins on PVDF were incubated with a rabbit anti-GFP antibody (1:5000) overnight at 4 °C and reacted with an HRP-conjugated goat anti-rabbit IgG (1:10,000, Jackson ImmunoResearch, West Grove, PA, U.S.A.) and LumiFlash Prime Chemiluminescent Substrate, HRP (Visual Protein, Taipei, Taiwan). .. Chemiluminescence signals were acquired using the ChemiDoc MP imaging system (Bio-Rad) and ImageLab software (version 6.1.0). ..

    Article Title: Multi-omic profiling reveals metabolic vulnerabilities in enzalutamide resistant prostate cancer
    Article Snippet: .. Equal protein amounts were resolved by SDS-PAGE (10% Mini-PROTEAN TGX, Bio-Rad), activated using stain-free imaging (ChemiDoc MP and ImageLab software Version 4.1, BioRad Laboratories), and transferred to PVDF membranes using a Trans-Blot Turbo system. .. Membranes were blocked in 5% skim milk/TBST or 5% BSA/ TBST and incubated overnight at 4 °C with anti-SREBP1 (BD Pharmingen, 557036; 1:500) or anti-CPS1 (Abcam, ab45956; 1:1 000).

    Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.
    Article Snippet: .. For protein detection Western Lightning Plus ECL solution (Perkin-Elmer, NEL105001EA) or Clarity Western ECL Substrate (Bio-Rad, 1705061) was used and analyzed with the ChemiDoc MP Imaging System and ImageLab 6.1 software (Bio-Rad). .. Quantification was done with ImageJ v.1.54 and normalized to β-actin.

    Software:

    Article Title: FANCB as a prognostic biomarker and therapeutic target in hepatocellular carcinoma with insights from pan-cancer analysis and experimental validation
    Article Snippet: .. Protein bands were visualized using the Bio-Rad ChemiDoc MP Imaging System and analyzed by Image J software. .. The primary antibodies used in this experiment include anti-FANCB rabbit polyclonal antibody (D263885, BBI Life Sciences Corporation, Shanghai, China) and GAPDH monoclonal antibody (60004-1-Ig, Proteintech, Wuhan, China).

    Article Title: Portulaca oleracea L.-derived extracellular vesicle-like particles reprogram intestinal ILCs to alleviate inflammatory bowel disease and colitis-associated colorectal cancer
    Article Snippet: .. The images were captured with the ChemiDoc MP Imaging System (USA, Bio-Rad) and analyzed with Image Lab Software. ..

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by ChemiDoc MP Imaging System (Bio-Rad). aSyn bands were identified and quantified using Image Lab software (Bio-Rad, RRID:SCR_014210), including normalization to total protein from stain-free labeling. ..

    Article Title: Dynamic Regulation of Atg4 Protease and Autophagy by Dithiothreitol and Iron in Alternaria alternata
    Article Snippet: Proteins on PVDF were incubated with a rabbit anti-GFP antibody (1:5000) overnight at 4 °C and reacted with an HRP-conjugated goat anti-rabbit IgG (1:10,000, Jackson ImmunoResearch, West Grove, PA, U.S.A.) and LumiFlash Prime Chemiluminescent Substrate, HRP (Visual Protein, Taipei, Taiwan). .. Chemiluminescence signals were acquired using the ChemiDoc MP imaging system (Bio-Rad) and ImageLab software (version 6.1.0). ..

    Article Title: Multi-omic profiling reveals metabolic vulnerabilities in enzalutamide resistant prostate cancer
    Article Snippet: .. Equal protein amounts were resolved by SDS-PAGE (10% Mini-PROTEAN TGX, Bio-Rad), activated using stain-free imaging (ChemiDoc MP and ImageLab software Version 4.1, BioRad Laboratories), and transferred to PVDF membranes using a Trans-Blot Turbo system. .. Membranes were blocked in 5% skim milk/TBST or 5% BSA/ TBST and incubated overnight at 4 °C with anti-SREBP1 (BD Pharmingen, 557036; 1:500) or anti-CPS1 (Abcam, ab45956; 1:1 000).

    Article Title: A ZEB1-Neon knock-in uncovers traceable dynamics of epithelial-mesenchymal transition in tumors in vivo.
    Article Snippet: .. For protein detection Western Lightning Plus ECL solution (Perkin-Elmer, NEL105001EA) or Clarity Western ECL Substrate (Bio-Rad, 1705061) was used and analyzed with the ChemiDoc MP Imaging System and ImageLab 6.1 software (Bio-Rad). .. Quantification was done with ImageJ v.1.54 and normalized to β-actin.

    Staining:

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by ChemiDoc MP Imaging System (Bio-Rad). aSyn bands were identified and quantified using Image Lab software (Bio-Rad, RRID:SCR_014210), including normalization to total protein from stain-free labeling. ..

    Article Title: Multi-omic profiling reveals metabolic vulnerabilities in enzalutamide resistant prostate cancer
    Article Snippet: .. Equal protein amounts were resolved by SDS-PAGE (10% Mini-PROTEAN TGX, Bio-Rad), activated using stain-free imaging (ChemiDoc MP and ImageLab software Version 4.1, BioRad Laboratories), and transferred to PVDF membranes using a Trans-Blot Turbo system. .. Membranes were blocked in 5% skim milk/TBST or 5% BSA/ TBST and incubated overnight at 4 °C with anti-SREBP1 (BD Pharmingen, 557036; 1:500) or anti-CPS1 (Abcam, ab45956; 1:1 000).

    Labeling:

    Article Title: Molecular profiling of alpha-synuclein pathology and seeding activity in Parkinson's disease.
    Article Snippet: Membranes were blocked for 1 h at RT in 10% non-fat dried milk added TBS-T (500 mM NaCl, 20 mM Tris, 0.1% Tween 20, pH 7.4) solution, incubated overnight at 4 °C with HRP anti-α-Synuclein (4B12, Biolegend) then washed with TBS-T solution and incubated for 1 h at RT with secondary antibodies (anti-biotin HRP-linked antibody (7075P5, Cell Signaling) or anti-mouse HRP-conjugated antibody (Goat anti-mouse Ig, Human ads-HRP, #1010-05, Southern Biotech)). .. Proteins were detected using an enhanced chemiluminescent detection system (ECL, MilliporeSigma Immobilon, #WBKLS0500) and imaged by ChemiDoc MP Imaging System (Bio-Rad). aSyn bands were identified and quantified using Image Lab software (Bio-Rad, RRID:SCR_014210), including normalization to total protein from stain-free labeling. ..

    SDS Page:

    Article Title: Multi-omic profiling reveals metabolic vulnerabilities in enzalutamide resistant prostate cancer
    Article Snippet: .. Equal protein amounts were resolved by SDS-PAGE (10% Mini-PROTEAN TGX, Bio-Rad), activated using stain-free imaging (ChemiDoc MP and ImageLab software Version 4.1, BioRad Laboratories), and transferred to PVDF membranes using a Trans-Blot Turbo system. .. Membranes were blocked in 5% skim milk/TBST or 5% BSA/ TBST and incubated overnight at 4 °C with anti-SREBP1 (BD Pharmingen, 557036; 1:500) or anti-CPS1 (Abcam, ab45956; 1:1 000).



    Similar Products

    99
    Bio-Rad chemidoc mp multimode imager
    Chemidoc Mp Multimode Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/Image+Lab+Touch+Software+for+ChemiDoc+Touch+Imager+Version+2%2E3/pmc13621846-284-6-10
    Average 99 stars, based on 1 article reviews
    chemidoc mp multimode imager - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    STATA Corporation stata mp 15 0 software
    Stata Mp 15 0 Software, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/STATA+15%2E0/pmc13019574-118-0-0
    Average 99 stars, based on 1 article reviews
    stata mp 15 0 software - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad chemidoc imaging software
    Chemidoc Imaging Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/ChemiDoc+MP+Imaging+System/10__1158_slash_2767___9764__crc___25___0701-104-57-60
    Average 99 stars, based on 1 article reviews
    chemidoc imaging software - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Refeyn Ltd refeyn discover mp software
    Refeyn Discover Mp Software, supplied by Refeyn Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/discover+mp+software/bio_rxiv__64898__2026__04__24__720727-202-6-6
    Average 86 stars, based on 1 article reviews
    refeyn discover mp software - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    99
    Bio-Rad chemidoc touch mp
    Chemidoc Touch Mp, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/Image+Lab+Touch+Software+for+ChemiDoc+Touch+Imager+Version+2%2E3/10__1093_slash_noajnl_slash_vdag101-66-5-8
    Average 99 stars, based on 1 article reviews
    chemidoc touch mp - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad image lab 5 0 software
    Image Lab 5 0 Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/ChemiDoc+MP+Imaging+System/bio_rxiv__64898__2026__04__06__715823-201-13-21
    Average 99 stars, based on 1 article reviews
    image lab 5 0 software - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Bio-Rad bioplex manager mp software
    Bioplex Manager Mp Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/Bio-Plex+Manager+Software/pm41897437-72-39-45
    Average 99 stars, based on 1 article reviews
    bioplex manager mp software - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    STATA Corporation stata mp version 14 software
    Stata Mp Version 14 Software, supplied by STATA Corporation, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/STATA+14%2E0/pm41888741-69-27-31
    Average 99 stars, based on 1 article reviews
    stata mp version 14 software - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    86
    Bionavis Inc mp spr navi data viewer 6 7 0 1 software
    <t>Label‐free</t> <t>MP‐SPR</t> quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.
    Mp Spr Navi Data Viewer 6 7 0 1 Software, supplied by Bionavis Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/0+1+6+7+data+mp+navi+software+spr+viewer/pmc13140515-200-25-31
    Average 86 stars, based on 1 article reviews
    mp spr navi data viewer 6 7 0 1 software - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    86
    Refeyn Ltd refeyn acquire mp software
    <t>Label‐free</t> <t>MP‐SPR</t> quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.
    Refeyn Acquire Mp Software, supplied by Refeyn Ltd, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/acquire+mp+refeyn+software/pm41792117-367-6-6
    Average 86 stars, based on 1 article reviews
    refeyn acquire mp software - by Bioz Stars, 2026-10
    86/100 stars
      Buy from Supplier

    Image Search Results


    Label‐free MP‐SPR quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.

    Journal: Journal of Extracellular Vesicles

    Article Title: Hyaluronic Acid Decoration Facilitates CD44‐Mediated Targeting and Alters Protein Corona Formation of Extracellular Vesicles

    doi: 10.1002/jev2.70263

    Figure Lengend Snippet: Label‐free MP‐SPR quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.

    Article Snippet: The SPR reflectance spectrum was recorded between 58° and 77° with a scanning time of approximately 2.0 s. Data were exported in ASCII format with MP‐SPR Navi Data Viewer 6.7.0.1 software (BioNavis), and SPR peak angular positions were determined using the weighted centroid method.

    Techniques: Comparison, Microscopy, Standard Deviation

    MP‐SPR based CD9‐antibody capture of EVs and plasma‐derived corona formation. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) and the ratio of the two wavelengths (dots) from CD9‐antibody captured MCF7‐EVs (A) and HAS3‐EVs (B) over 30‐min injections of EVs ( n = 3, STDEV). After EV capture, EV‐depleted plasma was injected to the system, causing a sharp increase in the SPR‐signal, followed by sharp decrease after washing with PBS (indicated with arrows). The corona refractive index and thickness were calculated from the remaining tail signal. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) for plasma injection and protein corona formation on MCF7‐EVs (C) and HAS3‐EVs (D) ( n = 3, STDEV). The average ratio of the signals measured at 670 and 785 nm for the dual‐wavelength method to calculate the EV diameters, and refractive index (RI) and thickness of the protein corona determined with LayerSolver are shown in the table. EV, extracellular vesicle; HA, hyaluronic acid.

    Journal: Journal of Extracellular Vesicles

    Article Title: Hyaluronic Acid Decoration Facilitates CD44‐Mediated Targeting and Alters Protein Corona Formation of Extracellular Vesicles

    doi: 10.1002/jev2.70263

    Figure Lengend Snippet: MP‐SPR based CD9‐antibody capture of EVs and plasma‐derived corona formation. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) and the ratio of the two wavelengths (dots) from CD9‐antibody captured MCF7‐EVs (A) and HAS3‐EVs (B) over 30‐min injections of EVs ( n = 3, STDEV). After EV capture, EV‐depleted plasma was injected to the system, causing a sharp increase in the SPR‐signal, followed by sharp decrease after washing with PBS (indicated with arrows). The corona refractive index and thickness were calculated from the remaining tail signal. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) for plasma injection and protein corona formation on MCF7‐EVs (C) and HAS3‐EVs (D) ( n = 3, STDEV). The average ratio of the signals measured at 670 and 785 nm for the dual‐wavelength method to calculate the EV diameters, and refractive index (RI) and thickness of the protein corona determined with LayerSolver are shown in the table. EV, extracellular vesicle; HA, hyaluronic acid.

    Article Snippet: The SPR reflectance spectrum was recorded between 58° and 77° with a scanning time of approximately 2.0 s. Data were exported in ASCII format with MP‐SPR Navi Data Viewer 6.7.0.1 software (BioNavis), and SPR peak angular positions were determined using the weighted centroid method.

    Techniques: Clinical Proteomics, Derivative Assay, Injection, Refractive Index