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chemidoc mp imager  (Bio-Rad)


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    Structured Review

    Bio-Rad chemidoc mp imager
    Chemidoc Mp Imager, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 2470 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mp+software/Image+Lab+Touch+Software+for+ChemiDoc+Touch+Imager+Version+2%2E3/pm42854292-95-11-14
    Average 99 stars, based on 2470 article reviews
    chemidoc mp imager - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Western Blot:

    Article Title: Expansion and optimization of the auxin-inducible degron 2 (AID2) system in Candida pathogens
    Article Snippet: Immunoblots using HRP were developed with Clarity Western ECL Substrate (Bio-Rad, 170-5060). .. All immunoblots were imaged on a ChemiDoc MP multimode imager (Bio-Rad). .. Band intensities were quantified using ImageLab software (Bio-Rad).

    Imaging:

    Article Title: Cyanidin-3-O-glucoside drives metabolic reprogramming and modulates PKA-dependent signaling in Leydig cells.
    Article Snippet: After extensive washing, membranes were exposed for 90 min to HRP-conjugated secondary antibody (Mouse anti-goat IgG-HRP, sc-2354, Santa Cruz Biotechnology, 1:5000 and Anti-Rabbit, AB6721, Abcam, 1:1000), and chemiluminescent detection was carried out using WesternBright ECL substrate (Advansta, USA). .. Images were acquired with a ChemiDoc MP Imaging System (Bio-Rad, Hercules, USA), and band intensities were quantified using Image Lab Software (v.6.0, Bio-Rad). ..

    Article Title: FANCB as a prognostic biomarker and therapeutic target in hepatocellular carcinoma with insights from pan-cancer analysis and experimental validation
    Article Snippet: .. Protein bands were visualized using the Bio-Rad ChemiDoc MP Imaging System and analyzed by Image J software. .. The primary antibodies used in this experiment include anti-FANCB rabbit polyclonal antibody (D263885, BBI Life Sciences Corporation, Shanghai, China) and GAPDH monoclonal antibody (60004-1-Ig, Proteintech, Wuhan, China).

    Article Title: Expression and role of SIRT1 and SIRT6 in porcine conceptus and trophoblasts: relevance to early placenta development†.
    Article Snippet: After washing in TBS-T, the membranes were incubated for 1 h with the anti-rabbit IgG-alkaline phosphatase antibody (A8025; 1:20,000; Sigma-Aldrich), and the immune complexes were visualized using the WESTAR ECL 2.0 kit (Cyanagen, Bologna, Italy). .. Images were captured in the ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, USA) and quantified using Image Lab 6 software (Bio-Rad). ..

    Article Title: Multi-omic profiling reveals metabolic vulnerabilities in enzalutamide resistant prostate cancer
    Article Snippet: .. Equal protein amounts were resolved by SDS-PAGE (10% Mini-PROTEAN TGX, Bio-Rad), activated using stain-free imaging (ChemiDoc MP and ImageLab software Version 4.1, BioRad Laboratories), and transferred to PVDF membranes using a Trans-Blot Turbo system. .. Membranes were blocked in 5% skim milk/TBST or 5% BSA/ TBST and incubated overnight at 4 °C with anti-SREBP1 (BD Pharmingen, 557036; 1:500) or anti-CPS1 (Abcam, ab45956; 1:1 000).

    Article Title: Structure-Guided Discovery of High-Affinity Methotrexate Aptamers for Electrochemical Drug Monitoring
    Article Snippet: .. FAM-labelled DNA bands were visualized using a ChemiDoc MP Imaging System (Bio-Rad) with an Alexa Fluor 488 filter, and band intensities corresponding to intact aptamers were quantified using Image Lab Software (Bio-Rad). ..

    Software:

    Article Title: Cyanidin-3-O-glucoside drives metabolic reprogramming and modulates PKA-dependent signaling in Leydig cells.
    Article Snippet: After extensive washing, membranes were exposed for 90 min to HRP-conjugated secondary antibody (Mouse anti-goat IgG-HRP, sc-2354, Santa Cruz Biotechnology, 1:5000 and Anti-Rabbit, AB6721, Abcam, 1:1000), and chemiluminescent detection was carried out using WesternBright ECL substrate (Advansta, USA). .. Images were acquired with a ChemiDoc MP Imaging System (Bio-Rad, Hercules, USA), and band intensities were quantified using Image Lab Software (v.6.0, Bio-Rad). ..

    Article Title: FANCB as a prognostic biomarker and therapeutic target in hepatocellular carcinoma with insights from pan-cancer analysis and experimental validation
    Article Snippet: .. Protein bands were visualized using the Bio-Rad ChemiDoc MP Imaging System and analyzed by Image J software. .. The primary antibodies used in this experiment include anti-FANCB rabbit polyclonal antibody (D263885, BBI Life Sciences Corporation, Shanghai, China) and GAPDH monoclonal antibody (60004-1-Ig, Proteintech, Wuhan, China).

    Article Title: Bird diversity and health status of bioindicator species (Coturnix coturnix, Horsfield, 1821) in Egypt's Manzala Lagoon: seasonal resilience monitoring.
    Article Snippet: Manzala Lagoon, the largest coastal wetland of Egypt, lies within the Nile Delta and serves as an essential sanctuary for both resident and migratory birds.. Despite its importance for regional biodiversity, the ecosystem faces significant anthropogenic pressures, with recent dredging activities constituting a major disturbance.. This study aimed to evaluate dredging impacts on bird diversity and environmental health in the Ashtoum El-Gamil Protected Area.

    Article Title: Expression and role of SIRT1 and SIRT6 in porcine conceptus and trophoblasts: relevance to early placenta development†.
    Article Snippet: After washing in TBS-T, the membranes were incubated for 1 h with the anti-rabbit IgG-alkaline phosphatase antibody (A8025; 1:20,000; Sigma-Aldrich), and the immune complexes were visualized using the WESTAR ECL 2.0 kit (Cyanagen, Bologna, Italy). .. Images were captured in the ChemiDoc Touch Imaging System (Bio-Rad, Hercules, CA, USA) and quantified using Image Lab 6 software (Bio-Rad). ..

    Article Title: Multi-omic profiling reveals metabolic vulnerabilities in enzalutamide resistant prostate cancer
    Article Snippet: .. Equal protein amounts were resolved by SDS-PAGE (10% Mini-PROTEAN TGX, Bio-Rad), activated using stain-free imaging (ChemiDoc MP and ImageLab software Version 4.1, BioRad Laboratories), and transferred to PVDF membranes using a Trans-Blot Turbo system. .. Membranes were blocked in 5% skim milk/TBST or 5% BSA/ TBST and incubated overnight at 4 °C with anti-SREBP1 (BD Pharmingen, 557036; 1:500) or anti-CPS1 (Abcam, ab45956; 1:1 000).

    Article Title: Structure-Guided Discovery of High-Affinity Methotrexate Aptamers for Electrochemical Drug Monitoring
    Article Snippet: .. FAM-labelled DNA bands were visualized using a ChemiDoc MP Imaging System (Bio-Rad) with an Alexa Fluor 488 filter, and band intensities corresponding to intact aptamers were quantified using Image Lab Software (Bio-Rad). ..

    Immunodetection:

    Article Title: Bird diversity and health status of bioindicator species (Coturnix coturnix, Horsfield, 1821) in Egypt's Manzala Lagoon: seasonal resilience monitoring.
    Article Snippet: Manzala Lagoon, the largest coastal wetland of Egypt, lies within the Nile Delta and serves as an essential sanctuary for both resident and migratory birds.. Despite its importance for regional biodiversity, the ecosystem faces significant anthropogenic pressures, with recent dredging activities constituting a major disturbance.. This study aimed to evaluate dredging impacts on bird diversity and environmental health in the Ashtoum El-Gamil Protected Area.

    SDS Page:

    Article Title: Multi-omic profiling reveals metabolic vulnerabilities in enzalutamide resistant prostate cancer
    Article Snippet: .. Equal protein amounts were resolved by SDS-PAGE (10% Mini-PROTEAN TGX, Bio-Rad), activated using stain-free imaging (ChemiDoc MP and ImageLab software Version 4.1, BioRad Laboratories), and transferred to PVDF membranes using a Trans-Blot Turbo system. .. Membranes were blocked in 5% skim milk/TBST or 5% BSA/ TBST and incubated overnight at 4 °C with anti-SREBP1 (BD Pharmingen, 557036; 1:500) or anti-CPS1 (Abcam, ab45956; 1:1 000).

    Staining:

    Article Title: Multi-omic profiling reveals metabolic vulnerabilities in enzalutamide resistant prostate cancer
    Article Snippet: .. Equal protein amounts were resolved by SDS-PAGE (10% Mini-PROTEAN TGX, Bio-Rad), activated using stain-free imaging (ChemiDoc MP and ImageLab software Version 4.1, BioRad Laboratories), and transferred to PVDF membranes using a Trans-Blot Turbo system. .. Membranes were blocked in 5% skim milk/TBST or 5% BSA/ TBST and incubated overnight at 4 °C with anti-SREBP1 (BD Pharmingen, 557036; 1:500) or anti-CPS1 (Abcam, ab45956; 1:1 000).



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    Bionavis Inc mp spr navi data viewer 6 7 0 1 software
    <t>Label‐free</t> <t>MP‐SPR</t> quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.
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    Image Search Results


    Label‐free MP‐SPR quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.

    Journal: Journal of Extracellular Vesicles

    Article Title: Hyaluronic Acid Decoration Facilitates CD44‐Mediated Targeting and Alters Protein Corona Formation of Extracellular Vesicles

    doi: 10.1002/jev2.70263

    Figure Lengend Snippet: Label‐free MP‐SPR quantification of MCF7‐EV and HAS3‐EV interactions with CD44 + and CD44 − cells. (A) Representative SPR curve comparison between blank gold sensor, fibronectin coated sensor, CD44 + monolayer, and CD44 − monolayer. Phase contrast microscopy images of the (B) CD44 + and (E) CD44 − cell monolayers before and after the SPR measurement. Scale bars correspond to 200 µm. MP‐SPR signal of the interaction between MCF7‐EVs and (C) CD44 + cells or (F) CD44 − cells. Interaction between HAS3‐EVs with (D) CD44 + cells or (G) CD44 − cells. SPR‐curves with standard deviation from two (HAS3‐EVs with CD44 + cells) to three biological replicate measurements are shown. EV, extracellular vesicle; HA, hyaluronic acid.

    Article Snippet: The SPR reflectance spectrum was recorded between 58° and 77° with a scanning time of approximately 2.0 s. Data were exported in ASCII format with MP‐SPR Navi Data Viewer 6.7.0.1 software (BioNavis), and SPR peak angular positions were determined using the weighted centroid method.

    Techniques: Comparison, Microscopy, Standard Deviation

    MP‐SPR based CD9‐antibody capture of EVs and plasma‐derived corona formation. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) and the ratio of the two wavelengths (dots) from CD9‐antibody captured MCF7‐EVs (A) and HAS3‐EVs (B) over 30‐min injections of EVs ( n = 3, STDEV). After EV capture, EV‐depleted plasma was injected to the system, causing a sharp increase in the SPR‐signal, followed by sharp decrease after washing with PBS (indicated with arrows). The corona refractive index and thickness were calculated from the remaining tail signal. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) for plasma injection and protein corona formation on MCF7‐EVs (C) and HAS3‐EVs (D) ( n = 3, STDEV). The average ratio of the signals measured at 670 and 785 nm for the dual‐wavelength method to calculate the EV diameters, and refractive index (RI) and thickness of the protein corona determined with LayerSolver are shown in the table. EV, extracellular vesicle; HA, hyaluronic acid.

    Journal: Journal of Extracellular Vesicles

    Article Title: Hyaluronic Acid Decoration Facilitates CD44‐Mediated Targeting and Alters Protein Corona Formation of Extracellular Vesicles

    doi: 10.1002/jev2.70263

    Figure Lengend Snippet: MP‐SPR based CD9‐antibody capture of EVs and plasma‐derived corona formation. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) and the ratio of the two wavelengths (dots) from CD9‐antibody captured MCF7‐EVs (A) and HAS3‐EVs (B) over 30‐min injections of EVs ( n = 3, STDEV). After EV capture, EV‐depleted plasma was injected to the system, causing a sharp increase in the SPR‐signal, followed by sharp decrease after washing with PBS (indicated with arrows). The corona refractive index and thickness were calculated from the remaining tail signal. SPR responses with 670 nm (line) and 785 nm lasers (dashed line) for plasma injection and protein corona formation on MCF7‐EVs (C) and HAS3‐EVs (D) ( n = 3, STDEV). The average ratio of the signals measured at 670 and 785 nm for the dual‐wavelength method to calculate the EV diameters, and refractive index (RI) and thickness of the protein corona determined with LayerSolver are shown in the table. EV, extracellular vesicle; HA, hyaluronic acid.

    Article Snippet: The SPR reflectance spectrum was recorded between 58° and 77° with a scanning time of approximately 2.0 s. Data were exported in ASCII format with MP‐SPR Navi Data Viewer 6.7.0.1 software (BioNavis), and SPR peak angular positions were determined using the weighted centroid method.

    Techniques: Clinical Proteomics, Derivative Assay, Injection, Refractive Index